lenticrisprv2 backbone Search Results


lenticrisprv2 backbone  (Addgene inc)
96
Addgene inc lenticrisprv2 backbone
Lenticrisprv2 Backbone, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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plenticrisprv2 backbones carrying neomycin  (Addgene inc)
96
Addgene inc plenticrisprv2 backbones carrying neomycin
Plenticrisprv2 Backbones Carrying Neomycin, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 96 stars, based on 1 article reviews
plenticrisprv2 backbones carrying neomycin - by Bioz Stars, 2026-10
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lenticrisprv2 blast backbone  (New England Biolabs)
99
New England Biolabs lenticrisprv2 blast backbone
Associations of MYC status versus response to PI3K/mTOR inhibition. (A) Comparison of MTOR , RPTOR , and RICTOR gene essentiality scores of cancer cell lines with and without MYC amplifications. Scores and copy number data were obtained from the DepMap (CRISPR, Public 22Q1) and the Cell Model Passports ( ; ) database, respectively. Red dotted line (−1) indicates the average gene essentiality scores of known essential genes. Data are represented as median ± IQR (box) and quartiles ± 1.5 × IQR (whiskers) and P values were computed with one-tailed Student’s t tests (*P < 0.05; **P < 0.01; n.s, not significant). (B) KEP1.23 cells overexpressing GFP or Myc were transfected with <t>lentiCRISPRv2-sgRNA-Cas9-Blast</t> vectors containing the indicated sgRNAs targeting Mtor , Rptor , or Rictor and selected with blasticidin. At days 2 (t1), 4 (t2), and 6 (t3) after blasticidin selection, cells were lysed and allele frequencies for the indicated genes were determined using TIDE . (C) Incucyte Live-Cell proliferation assay of KEP cell lines overexpressing GFP or Myc and treated with vehicle or 25 nM AZD8055 for 7 d. Data are represented as mean ± SEM of three replica per group of two independent experiments. (D) Dose–response curves of KEP cell lines overexpressing GFP or MYC , treated with alpelisib or buparlisib for 3 d, and measured by SRB colorimetric assay. Data are represented as mean ± standard deviation of five technical replicas per group of three independent experiments.
Lenticrisprv2 Blast Backbone, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/lenticrisprv2+backbone/BsmBI-v2/pmc10465700-232-1-11
Average 99 stars, based on 1 article reviews
lenticrisprv2 blast backbone - by Bioz Stars, 2026-10
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backbone lenticrisprv2  (Addgene inc)
93
Addgene inc backbone lenticrisprv2
Associations of MYC status versus response to PI3K/mTOR inhibition. (A) Comparison of MTOR , RPTOR , and RICTOR gene essentiality scores of cancer cell lines with and without MYC amplifications. Scores and copy number data were obtained from the DepMap (CRISPR, Public 22Q1) and the Cell Model Passports ( ; ) database, respectively. Red dotted line (−1) indicates the average gene essentiality scores of known essential genes. Data are represented as median ± IQR (box) and quartiles ± 1.5 × IQR (whiskers) and P values were computed with one-tailed Student’s t tests (*P < 0.05; **P < 0.01; n.s, not significant). (B) KEP1.23 cells overexpressing GFP or Myc were transfected with <t>lentiCRISPRv2-sgRNA-Cas9-Blast</t> vectors containing the indicated sgRNAs targeting Mtor , Rptor , or Rictor and selected with blasticidin. At days 2 (t1), 4 (t2), and 6 (t3) after blasticidin selection, cells were lysed and allele frequencies for the indicated genes were determined using TIDE . (C) Incucyte Live-Cell proliferation assay of KEP cell lines overexpressing GFP or Myc and treated with vehicle or 25 nM AZD8055 for 7 d. Data are represented as mean ± SEM of three replica per group of two independent experiments. (D) Dose–response curves of KEP cell lines overexpressing GFP or MYC , treated with alpelisib or buparlisib for 3 d, and measured by SRB colorimetric assay. Data are represented as mean ± standard deviation of five technical replicas per group of three independent experiments.
Backbone Lenticrisprv2, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/lenticrisprv2+backbone/PDIK1L+gRNA+(BRDN0001145465)+(Plasmid+%2376441)/10__1158_slash_1535___7163__mct___22___0004-55-16-32
Average 93 stars, based on 1 article reviews
backbone lenticrisprv2 - by Bioz Stars, 2026-10
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plenticrispr v2 puro backbone  (Addgene inc)
98
Addgene inc plenticrispr v2 puro backbone
Associations of MYC status versus response to PI3K/mTOR inhibition. (A) Comparison of MTOR , RPTOR , and RICTOR gene essentiality scores of cancer cell lines with and without MYC amplifications. Scores and copy number data were obtained from the DepMap (CRISPR, Public 22Q1) and the Cell Model Passports ( ; ) database, respectively. Red dotted line (−1) indicates the average gene essentiality scores of known essential genes. Data are represented as median ± IQR (box) and quartiles ± 1.5 × IQR (whiskers) and P values were computed with one-tailed Student’s t tests (*P < 0.05; **P < 0.01; n.s, not significant). (B) KEP1.23 cells overexpressing GFP or Myc were transfected with <t>lentiCRISPRv2-sgRNA-Cas9-Blast</t> vectors containing the indicated sgRNAs targeting Mtor , Rptor , or Rictor and selected with blasticidin. At days 2 (t1), 4 (t2), and 6 (t3) after blasticidin selection, cells were lysed and allele frequencies for the indicated genes were determined using TIDE . (C) Incucyte Live-Cell proliferation assay of KEP cell lines overexpressing GFP or Myc and treated with vehicle or 25 nM AZD8055 for 7 d. Data are represented as mean ± SEM of three replica per group of two independent experiments. (D) Dose–response curves of KEP cell lines overexpressing GFP or MYC , treated with alpelisib or buparlisib for 3 d, and measured by SRB colorimetric assay. Data are represented as mean ± standard deviation of five technical replicas per group of three independent experiments.
Plenticrispr V2 Puro Backbone, supplied by Addgene inc, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/lenticrisprv2+backbone/lentiCRISPRv2+puro+(Plasmid+%2398290)/pmc12075365-122-13-16
Average 98 stars, based on 1 article reviews
plenticrispr v2 puro backbone - by Bioz Stars, 2026-10
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lenticrisprv2 plasmid backbone  (Addgene inc)
93
Addgene inc lenticrisprv2 plasmid backbone
Associations of MYC status versus response to PI3K/mTOR inhibition. (A) Comparison of MTOR , RPTOR , and RICTOR gene essentiality scores of cancer cell lines with and without MYC amplifications. Scores and copy number data were obtained from the DepMap (CRISPR, Public 22Q1) and the Cell Model Passports ( ; ) database, respectively. Red dotted line (−1) indicates the average gene essentiality scores of known essential genes. Data are represented as median ± IQR (box) and quartiles ± 1.5 × IQR (whiskers) and P values were computed with one-tailed Student’s t tests (*P < 0.05; **P < 0.01; n.s, not significant). (B) KEP1.23 cells overexpressing GFP or Myc were transfected with <t>lentiCRISPRv2-sgRNA-Cas9-Blast</t> vectors containing the indicated sgRNAs targeting Mtor , Rptor , or Rictor and selected with blasticidin. At days 2 (t1), 4 (t2), and 6 (t3) after blasticidin selection, cells were lysed and allele frequencies for the indicated genes were determined using TIDE . (C) Incucyte Live-Cell proliferation assay of KEP cell lines overexpressing GFP or Myc and treated with vehicle or 25 nM AZD8055 for 7 d. Data are represented as mean ± SEM of three replica per group of two independent experiments. (D) Dose–response curves of KEP cell lines overexpressing GFP or MYC , treated with alpelisib or buparlisib for 3 d, and measured by SRB colorimetric assay. Data are represented as mean ± standard deviation of five technical replicas per group of three independent experiments.
Lenticrisprv2 Plasmid Backbone, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/lenticrisprv2+backbone/U371-HTR+(Plasmid+%2324469)/bio_rxiv__2025__06__16__659507-313-27-30
Average 93 stars, based on 1 article reviews
lenticrisprv2 plasmid backbone - by Bioz Stars, 2026-10
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pefs cas9 p2a puror  (Addgene inc)
93
Addgene inc pefs cas9 p2a puror
Associations of MYC status versus response to PI3K/mTOR inhibition. (A) Comparison of MTOR , RPTOR , and RICTOR gene essentiality scores of cancer cell lines with and without MYC amplifications. Scores and copy number data were obtained from the DepMap (CRISPR, Public 22Q1) and the Cell Model Passports ( ; ) database, respectively. Red dotted line (−1) indicates the average gene essentiality scores of known essential genes. Data are represented as median ± IQR (box) and quartiles ± 1.5 × IQR (whiskers) and P values were computed with one-tailed Student’s t tests (*P < 0.05; **P < 0.01; n.s, not significant). (B) KEP1.23 cells overexpressing GFP or Myc were transfected with <t>lentiCRISPRv2-sgRNA-Cas9-Blast</t> vectors containing the indicated sgRNAs targeting Mtor , Rptor , or Rictor and selected with blasticidin. At days 2 (t1), 4 (t2), and 6 (t3) after blasticidin selection, cells were lysed and allele frequencies for the indicated genes were determined using TIDE . (C) Incucyte Live-Cell proliferation assay of KEP cell lines overexpressing GFP or Myc and treated with vehicle or 25 nM AZD8055 for 7 d. Data are represented as mean ± SEM of three replica per group of two independent experiments. (D) Dose–response curves of KEP cell lines overexpressing GFP or MYC , treated with alpelisib or buparlisib for 3 d, and measured by SRB colorimetric assay. Data are represented as mean ± standard deviation of five technical replicas per group of three independent experiments.
Pefs Cas9 P2a Puror, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/lenticrisprv2+backbone/pUNIV-SIG-5HTR4D100A+(Plasmid+%2316312)/bio_rxiv__2025__10__07__681010-53-7-10
Average 93 stars, based on 1 article reviews
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backbone vector lenticrisprv2 mcherry  (Addgene inc)
95
Addgene inc backbone vector lenticrisprv2 mcherry
Associations of MYC status versus response to PI3K/mTOR inhibition. (A) Comparison of MTOR , RPTOR , and RICTOR gene essentiality scores of cancer cell lines with and without MYC amplifications. Scores and copy number data were obtained from the DepMap (CRISPR, Public 22Q1) and the Cell Model Passports ( ; ) database, respectively. Red dotted line (−1) indicates the average gene essentiality scores of known essential genes. Data are represented as median ± IQR (box) and quartiles ± 1.5 × IQR (whiskers) and P values were computed with one-tailed Student’s t tests (*P < 0.05; **P < 0.01; n.s, not significant). (B) KEP1.23 cells overexpressing GFP or Myc were transfected with <t>lentiCRISPRv2-sgRNA-Cas9-Blast</t> vectors containing the indicated sgRNAs targeting Mtor , Rptor , or Rictor and selected with blasticidin. At days 2 (t1), 4 (t2), and 6 (t3) after blasticidin selection, cells were lysed and allele frequencies for the indicated genes were determined using TIDE . (C) Incucyte Live-Cell proliferation assay of KEP cell lines overexpressing GFP or Myc and treated with vehicle or 25 nM AZD8055 for 7 d. Data are represented as mean ± SEM of three replica per group of two independent experiments. (D) Dose–response curves of KEP cell lines overexpressing GFP or MYC , treated with alpelisib or buparlisib for 3 d, and measured by SRB colorimetric assay. Data are represented as mean ± standard deviation of five technical replicas per group of three independent experiments.
Backbone Vector Lenticrisprv2 Mcherry, supplied by Addgene inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/lenticrisprv2+backbone/LentiCRISPRv2-mCherry+(Plasmid+%2399154)/bio_rxiv__64898__2025__12__04__691935-348-4-12
Average 95 stars, based on 1 article reviews
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lenticrisprv2 green fluorescent protein gfp backbone  (Addgene inc)
97
Addgene inc lenticrisprv2 green fluorescent protein gfp backbone
Associations of MYC status versus response to PI3K/mTOR inhibition. (A) Comparison of MTOR , RPTOR , and RICTOR gene essentiality scores of cancer cell lines with and without MYC amplifications. Scores and copy number data were obtained from the DepMap (CRISPR, Public 22Q1) and the Cell Model Passports ( ; ) database, respectively. Red dotted line (−1) indicates the average gene essentiality scores of known essential genes. Data are represented as median ± IQR (box) and quartiles ± 1.5 × IQR (whiskers) and P values were computed with one-tailed Student’s t tests (*P < 0.05; **P < 0.01; n.s, not significant). (B) KEP1.23 cells overexpressing GFP or Myc were transfected with <t>lentiCRISPRv2-sgRNA-Cas9-Blast</t> vectors containing the indicated sgRNAs targeting Mtor , Rptor , or Rictor and selected with blasticidin. At days 2 (t1), 4 (t2), and 6 (t3) after blasticidin selection, cells were lysed and allele frequencies for the indicated genes were determined using TIDE . (C) Incucyte Live-Cell proliferation assay of KEP cell lines overexpressing GFP or Myc and treated with vehicle or 25 nM AZD8055 for 7 d. Data are represented as mean ± SEM of three replica per group of two independent experiments. (D) Dose–response curves of KEP cell lines overexpressing GFP or MYC , treated with alpelisib or buparlisib for 3 d, and measured by SRB colorimetric assay. Data are represented as mean ± standard deviation of five technical replicas per group of three independent experiments.
Lenticrisprv2 Green Fluorescent Protein Gfp Backbone, supplied by Addgene inc, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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lcv2 hygro backbone  (Addgene inc)
94
Addgene inc lcv2 hygro backbone
Associations of MYC status versus response to PI3K/mTOR inhibition. (A) Comparison of MTOR , RPTOR , and RICTOR gene essentiality scores of cancer cell lines with and without MYC amplifications. Scores and copy number data were obtained from the DepMap (CRISPR, Public 22Q1) and the Cell Model Passports ( ; ) database, respectively. Red dotted line (−1) indicates the average gene essentiality scores of known essential genes. Data are represented as median ± IQR (box) and quartiles ± 1.5 × IQR (whiskers) and P values were computed with one-tailed Student’s t tests (*P < 0.05; **P < 0.01; n.s, not significant). (B) KEP1.23 cells overexpressing GFP or Myc were transfected with <t>lentiCRISPRv2-sgRNA-Cas9-Blast</t> vectors containing the indicated sgRNAs targeting Mtor , Rptor , or Rictor and selected with blasticidin. At days 2 (t1), 4 (t2), and 6 (t3) after blasticidin selection, cells were lysed and allele frequencies for the indicated genes were determined using TIDE . (C) Incucyte Live-Cell proliferation assay of KEP cell lines overexpressing GFP or Myc and treated with vehicle or 25 nM AZD8055 for 7 d. Data are represented as mean ± SEM of three replica per group of two independent experiments. (D) Dose–response curves of KEP cell lines overexpressing GFP or MYC , treated with alpelisib or buparlisib for 3 d, and measured by SRB colorimetric assay. Data are represented as mean ± standard deviation of five technical replicas per group of three independent experiments.
Lcv2 Hygro Backbone, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/lenticrisprv2+backbone/lentiCRISPRv2+hygro+(Plasmid+%2398291)/pmc12589105-374-36-38
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Image Search Results


Associations of MYC status versus response to PI3K/mTOR inhibition. (A) Comparison of MTOR , RPTOR , and RICTOR gene essentiality scores of cancer cell lines with and without MYC amplifications. Scores and copy number data were obtained from the DepMap (CRISPR, Public 22Q1) and the Cell Model Passports ( ; ) database, respectively. Red dotted line (−1) indicates the average gene essentiality scores of known essential genes. Data are represented as median ± IQR (box) and quartiles ± 1.5 × IQR (whiskers) and P values were computed with one-tailed Student’s t tests (*P < 0.05; **P < 0.01; n.s, not significant). (B) KEP1.23 cells overexpressing GFP or Myc were transfected with lentiCRISPRv2-sgRNA-Cas9-Blast vectors containing the indicated sgRNAs targeting Mtor , Rptor , or Rictor and selected with blasticidin. At days 2 (t1), 4 (t2), and 6 (t3) after blasticidin selection, cells were lysed and allele frequencies for the indicated genes were determined using TIDE . (C) Incucyte Live-Cell proliferation assay of KEP cell lines overexpressing GFP or Myc and treated with vehicle or 25 nM AZD8055 for 7 d. Data are represented as mean ± SEM of three replica per group of two independent experiments. (D) Dose–response curves of KEP cell lines overexpressing GFP or MYC , treated with alpelisib or buparlisib for 3 d, and measured by SRB colorimetric assay. Data are represented as mean ± standard deviation of five technical replicas per group of three independent experiments.

Journal: The Journal of Experimental Medicine

Article Title: MYC is a clinically significant driver of mTOR inhibitor resistance in breast cancer

doi: 10.1084/jem.20211743

Figure Lengend Snippet: Associations of MYC status versus response to PI3K/mTOR inhibition. (A) Comparison of MTOR , RPTOR , and RICTOR gene essentiality scores of cancer cell lines with and without MYC amplifications. Scores and copy number data were obtained from the DepMap (CRISPR, Public 22Q1) and the Cell Model Passports ( ; ) database, respectively. Red dotted line (−1) indicates the average gene essentiality scores of known essential genes. Data are represented as median ± IQR (box) and quartiles ± 1.5 × IQR (whiskers) and P values were computed with one-tailed Student’s t tests (*P < 0.05; **P < 0.01; n.s, not significant). (B) KEP1.23 cells overexpressing GFP or Myc were transfected with lentiCRISPRv2-sgRNA-Cas9-Blast vectors containing the indicated sgRNAs targeting Mtor , Rptor , or Rictor and selected with blasticidin. At days 2 (t1), 4 (t2), and 6 (t3) after blasticidin selection, cells were lysed and allele frequencies for the indicated genes were determined using TIDE . (C) Incucyte Live-Cell proliferation assay of KEP cell lines overexpressing GFP or Myc and treated with vehicle or 25 nM AZD8055 for 7 d. Data are represented as mean ± SEM of three replica per group of two independent experiments. (D) Dose–response curves of KEP cell lines overexpressing GFP or MYC , treated with alpelisib or buparlisib for 3 d, and measured by SRB colorimetric assay. Data are represented as mean ± standard deviation of five technical replicas per group of three independent experiments.

Article Snippet: The lentiCRISPRv2-Blast backbone was digested with the BsmBI-v2 restriction enzyme (#R0739; New England BioLabs) for 2 h at 55°C, and the correct band was isolated from a 1% agarose gel using the Isolate II PCR and Gel Kit (#BIO-52060; Bioline).

Techniques: Inhibition, Comparison, CRISPR, One-tailed Test, Transfection, Selection, Proliferation Assay, Colorimetric Assay, Standard Deviation